Yourgene Well being (a part of the Novacyt group of firms), a number one worldwide molecular diagnostics group, and PacBio, a number one developer of high-quality, extremely correct sequencing options, at this time introduced a collaboration to optimize dimension choice for long-read sequencing workflows. PacBio has certified Yourgene’s LightBench^® instrument for dimension choice of lengthy DNA fragments, enabling excessive yields for HiFi sequencing knowledge.

Yourgene’s LightBench is an automatic electrophoresis instrument, powered by Ranger^® Expertise. This platform makes use of a patent-protected, machine-vision algorithm to interpret and management gel electrophoresis in actual time. It delivers correct dimension choice for human genomic DNA and consists of the power to carry out fragment size evaluation for extra high quality management.

PacBio HiFi sequencing is a single-molecule real-time (SMRT^®) sequencing know-how. It produces lengthy (1,000 to twenty,000+ bases), correct reads with uniform genomic protection and native methylation detection to be used in functions comparable to entire genome reconstruction, haplotype phasing, detection of huge and complicated variants, and epigenetics.

A brand new Technical Be aware was launched at this time for PacBio clients titled “Dimension choice of PacBio SMRTbell^® libraries with the LightBench^® instrument from Yourgene Well being.” The Yourgene LightBench instrument is suitable with the Revio system. The automated LightBench workflow optimizes stroll away time, providing a scalable dimension choice resolution for the preparation of PacBio SMRTbell sequencing libraries. Outcomes from the preliminary research assessing two fragment lengths in 84 human genomic DNA samples from frozen blood for library preparation confirmed constant yields for 8 kb or 10 kb dimension and a zero p.c failure price.

PacBio HiFi sequencing know-how is a recreation changer for entire genome functions. Excessive-resolution dimension choice is essential to maximizing knowledge yields from long-read sequencing and Yourgene is proud to supply researchers a exact and scalable resolution with the LightBench.”

Matthew Nesbitt, Co-Founding father of Ranger Expertise, Yourgene Well being